Differential expression of three different prepro‐GnRH (gonadotrophin‐releasing hormone) messengers in the brain of the european sea bass (Dicentrarchus labrax)
Identifieur interne : 000166 ( France/Analysis ); précédent : 000165; suivant : 000167Differential expression of three different prepro‐GnRH (gonadotrophin‐releasing hormone) messengers in the brain of the european sea bass (Dicentrarchus labrax)
Auteurs : David González-Martínez [Espagne, France] ; Thierry Madigou [France] ; Nilli Zmora [Israël] ; Isabelle Anglade [France] ; Silvia Zanuy [Espagne] ; Yonathan Zohar [Israël] ; Abigail Elizur [Israël] ; José Antonio Mu Oz-Cueto [Espagne] ; Olivier Kah [France]Source :
- Journal of Comparative Neurology [ 0021-9967 ] ; 2001-01-01.
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Abstract
The expression sites of three prepro‐gonadotrophin‐releasing hormones (GnRHs), corresponding to seabream GnRH (sbGnRH: Ser8‐mGnRH, mammalian GnRH), salmon GnRH (sGnRH: Trp7Leu8‐mGnRH), and chicken GnRH‐II (cGnRH‐II: His5Trp7Tyr8‐mGnRH) forms were studied in the brain of a perciform fish, the European sea bass (Dicentrarchus labrax) by means of in situ hybridization. The riboprobes used in this study correspond to the three GnRH‐associated peptide (GAP)‐coding regions of the prepro‐GnRH cDNAs cloned from the same species (salmon GAP: sGAP; seabream GAP: sbGAP; chicken GAP‐II: cIIGAP), which show little oligonucleotide sequence identity (sGAP versus sbGAP: 42%; cIIGAP versus sbGAP: 36%; sGAP versus cIIGAP: 41%). Adjacent paraffin sections (6 mm) throughout the entire brain were treated in parallel with each of the three anti‐sense probes and the corresponding sense probes, demonstrating the high specificity of the hybridization signal. The results showed that both sGAP and sbGAP mRNAs had a broader expression in the olfactory bulbs, ventral telencephalon, and preoptic region, whereas cIIGAP mRNA expression was confined to large cells of the nucleus of the medial longitudinal fascicle. In the olfactory bulbs, both the signal intensity and the number of positive cells were higher with the sGAP probe, whereas sbGAP mRNA‐expressing cells were more numerous and intensely stained in the preoptic region. Additional isolated sbGAP‐positive cells were detected in the ventrolateral hypothalamus. These results demonstrate a clear overlapping of sGAP‐ and sbGAP‐expressing cells in the forebrain of the European sea bass, in contrast to previous reports in other perciforms showing a clear segregation of these two cell populations. J. Comp. Neurol. 429:144–155, 2001. © 2000 Wiley‐Liss, Inc.
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DOI: 10.1002/1096-9861(20000101)429:1<144::AID-CNE11>3.0.CO;2-B
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The riboprobes used in this study correspond to the three GnRH‐associated peptide (GAP)‐coding regions of the prepro‐GnRH cDNAs cloned from the same species (salmon GAP: sGAP; seabream GAP: sbGAP; chicken GAP‐II: cIIGAP), which show little oligonucleotide sequence identity (sGAP versus sbGAP: 42%; cIIGAP versus sbGAP: 36%; sGAP versus cIIGAP: 41%). Adjacent paraffin sections (6 mm) throughout the entire brain were treated in parallel with each of the three anti‐sense probes and the corresponding sense probes, demonstrating the high specificity of the hybridization signal. The results showed that both sGAP and sbGAP mRNAs had a broader expression in the olfactory bulbs, ventral telencephalon, and preoptic region, whereas cIIGAP mRNA expression was confined to large cells of the nucleus of the medial longitudinal fascicle. In the olfactory bulbs, both the signal intensity and the number of positive cells were higher with the sGAP probe, whereas sbGAP mRNA‐expressing cells were more numerous and intensely stained in the preoptic region. Additional isolated sbGAP‐positive cells were detected in the ventrolateral hypothalamus. These results demonstrate a clear overlapping of sGAP‐ and sbGAP‐expressing cells in the forebrain of the European sea bass, in contrast to previous reports in other perciforms showing a clear segregation of these two cell populations. J. Comp. Neurol. 429:144–155, 2001. © 2000 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>Espagne</li><li>France</li><li>Israël</li></country><region><li>Andalousie</li><li>Région Bretagne</li></region><settlement><li>Rennes</li></settlement></list><tree><country name="Espagne"><region name="Andalousie"><name sortKey="Gonzalez Artinez, David" sort="Gonzalez Artinez, David" uniqKey="Gonzalez Artinez D" first="David" last="González-Martínez">David González-Martínez</name></region><name sortKey="Mu Oz Ueto, Jose Antonio" sort="Mu Oz Ueto, Jose Antonio" uniqKey="Mu Oz Ueto J" first="José Antonio" last="Mu Oz-Cueto">José Antonio Mu Oz-Cueto</name><name sortKey="Zanuy, Silvia" sort="Zanuy, Silvia" uniqKey="Zanuy S" first="Silvia" last="Zanuy">Silvia Zanuy</name></country><country name="France"><region name="Région Bretagne"><name sortKey="Gonzalez Artinez, David" sort="Gonzalez Artinez, David" uniqKey="Gonzalez Artinez D" first="David" last="González-Martínez">David González-Martínez</name></region><name sortKey="Anglade, Isabelle" sort="Anglade, Isabelle" uniqKey="Anglade I" first="Isabelle" last="Anglade">Isabelle Anglade</name><name sortKey="Kah, Olivier" sort="Kah, Olivier" uniqKey="Kah O" first="Olivier" last="Kah">Olivier Kah</name><name sortKey="Kah, Olivier" sort="Kah, Olivier" uniqKey="Kah O" first="Olivier" last="Kah">Olivier Kah</name><name sortKey="Madigou, Thierry" sort="Madigou, Thierry" uniqKey="Madigou T" first="Thierry" last="Madigou">Thierry Madigou</name></country><country name="Israël"><noRegion><name sortKey="Zmora, Nilli" sort="Zmora, Nilli" uniqKey="Zmora N" first="Nilli" last="Zmora">Nilli Zmora</name></noRegion><name sortKey="Elizur, Abigail" sort="Elizur, Abigail" uniqKey="Elizur A" first="Abigail" last="Elizur">Abigail Elizur</name><name sortKey="Zohar, Yonathan" sort="Zohar, Yonathan" uniqKey="Zohar Y" first="Yonathan" last="Zohar">Yonathan Zohar</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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